Journal article
Coassembled nanostructured bioscaffold reduces the expression of proinflammatory cytokines to induce apoptosis in epithelial cancer cells
R Li, S Pavuluri, K Bruggeman, BM Long, AJ Parnell, A Martel, SR Parnell, FM Pfeffer, AJC Dennison, KR Nicholas, CJ Barrow, DR Nisbet, RJ Williams
Nanomedicine Nanotechnology Biology and Medicine | ELSEVIER | Published : 2016
Abstract
A promising class of materials for such applications is the nanostructured scaffolds formed by epitope presenting minimalist self-assembled peptides; these are bioactive on a cellular length scale, while presenting as an easily handled hydrogel. Here, we show that the assembly process can distribute an anti-inflammatory polysaccharide, fucoidan, localized to the nanofibers within the scaffold to create a biomaterial for cancer therapy. We show that it supports healthy cells, while inducing apoptosis in cancerous epithelial cells, as demonstrated by the significant down-regulation of gene and protein expression pathways associated with epithelial cancer progression. Our findings highlight an ..
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Funding Acknowledgements
This work was funded by an Australian Research Council (ARC) Discovery Project (DP130103131). N.M.R. instrumentation was provided through ARC funding (LE110100141). D.R.N. was supported by an NHMRC Career Development Fellowship (APP1050684). R.J.W. was supported via an Alfred Deakin Research Fellowship. Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is gratefully acknowledged. A.J.C.D. was supported by the Swedish Research Council VR. Preliminary SANS measurements were performed at the Low Energy Neutron Source, Indiana. Access to the D33 instrument was provided by the Institut Laue-Langevin user access programme (8-03-826).